Apple's 9th retail store opened at 10am on Saturday in University Avenue, Palo Alto, California.
Apple CEO Steve Jobs attended the opening, and welcomed the crowds waiting outside the Bay Area Retail store.
The Palo Alto store is similar to the eight preceding it. However, there have been changes to pictures and the islands - display stands - holding peripherals, according to reports from MacCentral.
Ron Johnson, Apple's senior vice president of retail, said: "We're here to expand Apple's marketshare. The store is orientated to those 95 per cent that don't use Macs."
Johnson added that Apple still has plans to open a further 16 stores across the US by the end of the year, hiring 675 employees.
The next stores will open on October 13 in Germantown, Tennessee, and Albany, New York.