The bays will carry up to 30 Apple and third-party Mac software titles that will be updated quarterly.
The scheme is "set to launch in mid-May". Content will include games and reference titles, utility and system apps including Mac OS X 10.1 and Mac applications such as Corel Draw 10.
A prototype of the stand was placed in PC World Luton just after Christmas. Its success encouraged Apple and PC World to include the bay in all stores nationwide.
A regional rollout will start in London and the South including PC World's flagship store in Staples Corner, north London.
Tom Guy, marketing manager for software at PC World told Macworld: "We're trying to help customers shopping that area. It used to be difficult to shop for Mac software as it was originally on the same shelving as PCs.
"It was hard to differentiate Mac from PC software. There was also the added confusion that a high percentage of our selection are hybrid titles – they work on Mac and PC.
"This muddle brought Apple and PC World together some months ago. We sat down with Apple and undertook a project to segment the software in stores. We measured the success of the prototype stand thorough feedback via market research."
"There are more plans in the pipeline, " Guy added.